| STUDYID | STOREDB:STUDY1000 |
|---|---|
| CREATEDON | 2015-12-08 19:39:42 |
| MODIFIEDON | 2020-10-08 15:46:25 |
| UPLOADER | Omid Azimzadeh |
| DOI | DOI:10.20348/STOREDB/1000 |
| STUDY NAME | ||
|---|---|---|
| Integrative cross-omics analysis in cardiac endothelial cells unravels mechanisms of long-term radiation-induced vascular dysfunction | ||
| STUDY STATUS | ||
| Published: Open access to everyone | ||
| DATA SHARING POLICY | ||
| CC-Attribution | ||
| COUNTRY | ||
| Germany | ||
| PRINCIPAL INVESTIGATOR | ||
| Dr. Omid Azimzadeh | ||
| SPECIES | ||
| Mus musculus | ||
| BIOLOGICAL SAMPLE AVAILABLE | ||
| No | ||
| STUDY DESCRIPTION | ||
| Epidemiological data from radiotherapy patients show the damaging effect of ionising radiation on heart and vasculature. The endothelium is the main target of radiation damage and contributes essentially to the development of cardiac injury. However, the molecular mechanisms behind the radiation-induced endothelial dysfunction are not fully understood. In the present study, 10-week old C57Bl/6 mice received local X-ray heart doses of 8 or 16 Gy and were sacrificed after 16 weeks; the controls were sham-irradiated. The cardiac microvascular endothelial cells were isolated from the heart tissue using streptavidin-CD31 coated micro-beads. The cells were lysed and proteins were labelled with duplex isotope -coded protein label methodology for quantification. All samples were analysed by LC-ESI-MS/MS and Proteome Discoverer software. The proteomics data were further studied by bioinformatics tools and validated by targeted transcriptomics, immunoblotting, immunohistochemistry, and serum profiling. Radiation-induced endothelial dysfunction was characterised by impaired energy metabolism and perturbation of the insulin/IGF-PI3K-Akt signalling pathway. The data also strongly suggested premature endothelial senescence, increased oxidative stress, decreased NO availability and enhanced inflammation as main causes of radiation-induced long-term vascular dysfunction. Detailed data on molecular mechanisms of radiation-induced vascular injury as compiled here are essential in developing radiotherapy strategies that minimise cardiovascular complications. | ||
| DATASET NAME | ||
|---|---|---|
| cardiac EC_Highdose_IR | ||
| DOI | DOI:10.20348/STOREDB/1000/1000 | |
| DATASET DESCRIPTION | ||
| MS/MS data | ||
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