STOREDB:STUDY1000 Integrative cross-omics analysis in cardiac endothelial cells unravels mechanisms of long-term radiation-induced vascular dysfunction [DOI:10.20348/STOREDB/1000]

Study meta-data


STUDYIDSTOREDB:STUDY1000
CREATEDON2015-12-08 19:39:42
MODIFIEDON2020-10-08 15:46:25
UPLOADEROmid Azimzadeh
DOIDOI:10.20348/STOREDB/1000

Study details


STUDY NAME
Integrative cross-omics analysis in cardiac endothelial cells unravels mechanisms of long-term radiation-induced vascular dysfunction
STUDY STATUS
Published: Open access to everyone
DATA SHARING POLICY
CC-Attribution
COUNTRY
Germany
PRINCIPAL INVESTIGATOR
Dr. Omid Azimzadeh
SPECIES
Mus musculus
BIOLOGICAL SAMPLE AVAILABLE
No
STUDY DESCRIPTION
Epidemiological data from radiotherapy patients show the damaging effect of ionising radiation on heart and vasculature. The endothelium is the main target of radiation damage and contributes essentially to the development of cardiac injury. However, the molecular mechanisms behind the radiation-induced endothelial dysfunction are not fully understood. In the present study, 10-week old C57Bl/6 mice received local X-ray heart doses of 8 or 16 Gy and were sacrificed after 16 weeks; the controls were sham-irradiated. The cardiac microvascular endothelial cells were isolated from the heart tissue using streptavidin-CD31 coated micro-beads. The cells were lysed and proteins were labelled with duplex isotope -coded protein label methodology for quantification. All samples were analysed by LC-ESI-MS/MS and Proteome Discoverer software. The proteomics data were further studied by bioinformatics tools and validated by targeted transcriptomics, immunoblotting, immunohistochemistry, and serum profiling. Radiation-induced endothelial dysfunction was characterised by impaired energy metabolism and perturbation of the insulin/IGF-PI3K-Akt signalling pathway. The data also strongly suggested premature endothelial senescence, increased oxidative stress, decreased NO availability and enhanced inflammation as main causes of radiation-induced long-term vascular dysfunction. Detailed data on molecular mechanisms of radiation-induced vascular injury as compiled here are essential in developing radiotherapy strategies that minimise cardiovascular complications.

STOREDB:DATASET1000 cardiac EC_Highdose_IR [DOI:10.20348/STOREDB/1000/1000]


Created on:2015-12-08 19:40:22 Modified On:2015-12-09 18:31:51
DATASET NAME
cardiac EC_Highdose_IR
DOIDOI:10.20348/STOREDB/1000/1000
DATASET DESCRIPTION
MS/MS data
STOREDB:FILE12114

16gy_rep3_lane2_slice5

Uploaded on: 2020-10-08 15:39:52
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12113

16gy_rep3_lane2_slice4

Uploaded on: 2020-10-08 15:35:42
Type: image/x-panasonic-raw
File size: 887 megabytes
STOREDB:FILE12112

16gy_rep3_lane2_slice2

Uploaded on: 2020-10-08 15:23:44
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12111

16gy_rep3_lane2_slice3

Uploaded on: 2020-10-08 15:23:15
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12110

16gy_rep2_lane1_slice5

Uploaded on: 2020-10-08 15:09:09
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12109

16gy_rep3_lane2_slice1

Uploaded on: 2020-10-08 15:08:10
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12108

16gy_rep2_lane1_slice4

Uploaded on: 2020-10-08 14:53:25
Type: image/x-panasonic-raw
File size: 888 megabytes
STOREDB:FILE12107

16gy_rep2_lane1_slice3

Uploaded on: 2020-10-08 14:50:51
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12106

16gy_rep2_lane1_slice2

Uploaded on: 2020-10-08 14:40:34
Type: image/x-panasonic-raw
File size: 983 megabytes
STOREDB:FILE12105

16gy_rep2_lane1_slice1

Uploaded on: 2020-10-08 14:36:28
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12104

16gy_rep1_lane2_slice5

Uploaded on: 2020-10-08 14:26:18
Type: image/x-panasonic-raw
File size: 654 megabytes
STOREDB:FILE12103

16gy_rep1_lane2_slice4

Uploaded on: 2020-10-08 14:21:40
Type: image/x-panasonic-raw
File size: 593 megabytes
STOREDB:FILE12102

8gy_rep3_lnae1_slice5

Uploaded on: 2020-10-08 14:17:01
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12101

16gy_rep1_lane2_slice3

Uploaded on: 2020-10-08 14:13:12
Type: image/x-panasonic-raw
File size: 608 megabytes
STOREDB:FILE12100

16gy_rep1_lane2_slice2

Uploaded on: 2020-10-08 14:04:24
Type: image/x-panasonic-raw
File size: 594 megabytes
STOREDB:FILE12099

16gy_rep1_lane2_slice1

Uploaded on: 2020-10-08 13:38:42
Type: image/x-panasonic-raw
File size: 622 megabytes
STOREDB:FILE12098

8gy_rep3_lnae1_slice4

Uploaded on: 2020-10-08 13:29:54
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12097

8gy_rep3_lnae1_slice2

Uploaded on: 2020-10-08 13:18:31
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12096

8gy_rep3_lnae1_slice1

Uploaded on: 2020-10-08 13:14:55
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12095

8gy_rep_lnae1_slice3

Uploaded on: 2020-10-08 13:02:01
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12094

8gy_rep2_lane1_slice5

Uploaded on: 2020-10-08 12:59:40
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12093

8gy_rep2_lane1_slice4

Uploaded on: 2020-10-08 12:44:52
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12092

8gy_rep2_lane1_slice3

Uploaded on: 2020-10-08 12:40:07
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12091

8gy_rep2_lane1_slice2

Uploaded on: 2020-10-08 12:28:57
Type: image/x-panasonic-raw
File size: 1 gigabytes
STOREDB:FILE12090

8gy_rep1_lane1_slice5

Uploaded on: 2020-10-08 12:22:55
Type: image/x-panasonic-raw
File size: 778 megabytes
STOREDB:FILE12089

8gy_rep2_lane1_slice1

Uploaded on: 2020-10-08 12:12:49
Type: image/x-panasonic-raw
File size: 54 megabytes
STOREDB:FILE12088

8gy_rep1_lane1_slice4

Uploaded on: 2020-10-08 12:12:02
Type: image/x-panasonic-raw
File size: 596 megabytes
STOREDB:FILE12087

8gy_rep1_lane1_slice3

Uploaded on: 2020-10-08 12:11:55
Type: image/x-panasonic-raw
File size: 633 megabytes
STOREDB:FILE12086

8gy_rep1_lane1_slice1

Uploaded on: 2020-10-08 12:03:29
Type: image/x-panasonic-raw
File size: 646 megabytes
STOREDB:FILE12085

8gy_rep1_lane1_slice2

Uploaded on: 2020-10-08 12:02:55
Type: image/x-panasonic-raw
File size: 617 megabytes