STUDYID | STOREDB:STUDY1062 |
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CREATEDON | 2016-12-12 15:26:54 |
MODIFIEDON | 2016-12-12 15:34:21 |
UPLOADER | Katalin Lumniczky |
DOI | DOI:10.20348/STOREDB/1062 |
STUDY NAME | ||
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miRNA profile of extracellular vesicles isolated from bone marrow following in vivo irradiation | ||
STUDY STATUS | ||
Restricted: Downloaders have to ask uploader for file access | ||
COUNTRY | ||
Hungary | ||
PRINCIPAL INVESTIGATOR | ||
Katalin Lumniczky Ph.D. | ||
SPECIES | ||
Mus musculus | ||
SIZE OF COHORT | ||
0-999 | ||
RADONORM RESEARCH PRIORITY | ||
Analysis of mechanisms involved in low dose radiation through use and development of suitable cellular and animal models | ||
EXPOSURE CONTEXT | ||
Medical | ||
INTERNAL OR EXTERNAL EXPOSURE | ||
External | ||
TYPE OF EXTERNAL EXPOSURE | ||
X-ray | ||
AGE AT EXPOSURE | ||
Adult | ||
EXPOSURE PATTERN | ||
Acute | ||
DOSE RATE | ||
High | ||
BIOLOGICAL SAMPLE AVAILABLE | ||
No | ||
STUDY DESCRIPTION | ||
Male C57Bl/6 mice were irradiated with low and high dose X-ray irradiation. Mice were sacrificed 24 hours later and bone marrow-derived extracellular vesicles were isolated and microRNAs prepared. A miRNA profiling was performed by Exiqon (Vedbaek, Denmark), using qPCR panel with the miRNA Ready-to-Use PCR, Mouse&Rat panel I+II using ExiLENT SYBR® Green master mix. The amplification was performed in a LightCycler® 480 Real-Time PCR System (Roche) in 384 well plates The amplification curves were analysed using the Roche LC software, both for determination of quantification cycles (Cq) (by the 2nd derivative method) and for melting curve (Tm) analysis. |
DATASET NAME | ||
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Raw data microRNA from EV_Szatmari et al | ||
DOI | DOI:10.20348/STOREDB/1062/1101 | |
DATASET DESCRIPTION | ||
miRNA profiling of EVs isolated of bone marrow of whole body irradiated mice. Raw Cq-s of a microRNA qPCR panel are shown.Three experiments were performed (rows A; B; and C), with control (0 Gy), 0.1 Gy and 2 Gy doses. |
Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
File size: 94 kilobytes
Uploaded on: 2016-12-13 17:33:30
DATASET NAME | ||
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Raw data microRNA from EV_Szatmari et al | ||
DOI | DOI:10.20348/STOREDB/1062/1100 | |
DATASET DESCRIPTION | ||
Raw Cq values of miRNA qPCR panel analysis. Three set of samples were profiled: control, irradiated with 0.1 Gy and 2 Gy. Three independent experiments were performed(A-C). |