|Liver Tumours and Diseases in Rats After Fractionated Neutron Exposure or Injection of Non-radioactive Zirconotrast
|Published: Open access to everyone
|BIOLOGICAL SAMPLE AVAILABLE
|Purpose: To determine the respective roles of the radioactive and chemical component in Thorotrast induced liver damage. The fractionated neutron exposure was to simulate the radioactive, the Zirkonotrast the chemical damage from Thorotrast.
Status: 1986-1989, terminated
Treatment: Fifty fractions of 0.2 Gy of neutrons (14 MeV neutrons produced by deuterium-tritium reaction, dose rate 0.1 Gy/min) at intervals of 14 days (total 10 Gy) i.v. of 120 μl of Zirkonotrast (Zr-dioxide 13% w/v)
Dosimetry: Activity injected, twin ionisation chamber with tissue equivalent material
Endpoints: Serial killing (6 and 12 months) and life-span (spontaneous death) with macroscopic/microscopic pathology
Animal: Female Wistar rats aged 3-4 months at the start of the irradiation
Results: One year after the beginning of neutron irradiation, the first liver tumours were detected. At the end of the study after almost three years, the incidence of irradiated animals with liver tumours was about 40%. The animals treated additionally with Zirconotrast displayed nearly the same incidence, time of onset and overall number of liver tumours indicating that the fractionated neutron exposure was the exclusive cause of tumour development. Zirconotrast had no tumour promoting or tumour inducing effect. In comparison to earlier animal studies with Thorotrast, the same histological types of benign and malignant liver tumours were found.
FFPE Blocks are available at HMGU.
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