STUDYID | STOREDB:STUDY1104 |
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CREATEDON | 2018-02-07 13:44:44 |
MODIFIEDON | 2018-02-07 13:44:44 |
UPLOADER | Omid Azimzadeh |
DOI | DOI:10.20348/STOREDB/1104 |
STUDY NAME | ||
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Quantitative proteomic analysis reveals induction of premature senescence in human umbilical vein endothelial cells exposed to chronic low-dose rate gamma-radiation | ||
STUDY STATUS | ||
Published: Open access to everyone | ||
DATA SHARING POLICY | ||
CC-Attribution Non-Commercial Share Alike | ||
COUNTRY | ||
Germany | ||
PRINCIPAL INVESTIGATOR | ||
Dr.Soile Tapio | ||
SPECIES | ||
Homo sapiens | ||
SIZE OF COHORT | ||
0-999 | ||
OUTCOME | ||
Cardiovascular | ||
RADONORM RESEARCH PRIORITY | ||
Analysis of mechanisms involved in low dose radiation through use and development of suitable cellular and animal models | ||
EXPOSURE CONTEXT | ||
Occupational | ||
INTERNAL OR EXTERNAL EXPOSURE | ||
External | ||
TYPE OF EXTERNAL EXPOSURE | ||
gamma | ||
RADIONUCLIDE | ||
caesium | ||
AGE AT EXPOSURE | ||
Adult | ||
EXPOSURE PATTERN | ||
Transitory | ||
DOSE RATE | ||
Low | ||
BIOLOGICAL SAMPLE AVAILABLE | ||
No | ||
STUDY DESCRIPTION | ||
Chronic low-dose ionizing radiation induces cardiovascular disease in human populations but the mechanism is largely unknown. We suggested that chronic radiation exposure may induce endothelial cell senescence that is associated with vascular damage in vivo. We investigated whether chronic radiation exposure is causing a change in the onset of senescence in endothelial cells in vitro. Indeed, when exposed to continuous low-dose rate gamma radiation (4.1 mGy/h) primary human umbilical vein endothelial cells (HUVECs) initiated senescence much earlier than the non-irradiated control cells. We investigated the changes in the protein expression of HUVECs before and during the onset of radiation-induced senescence. Cellular proteins were quantified using isotope-coded protein label technology after 1, 3, and 6 weeks of radiation exposure. Several senescence-related biological pathways were influenced by radiation, including cytoskeletal organisation, cell-cell communication and adhesion, and inflammation. Immunoblot analysis showed an activation of the p53/p21 pathway corresponding to the progressing senescence. Our data suggest that chronic radiation-induced DNA damage and oxidative stress result in induction of p53/p21 pathway that inhibits the replicative potential of HUVECs and leads to premature senescence. This study contributes to the understanding of the increased risk of cardiovascular diseases seen in populations exposed to chronic low-dose irradiation. | ||
PUBMED ID | ||
23349028 | ||
MEAN DURATION OF FOLLOW-UP (WEEKS) | ||
12 |
DATASET NAME | ||
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continuous low-dose rate gamma irradiation (4.1 mGy/h) of primary human umbilical vein endothelial cells (HUVECs) | ||
DOI | DOI:10.20348/STOREDB/1104/1155 | |
LINK TO FILE | ||
http://onlinelibrary.wiley.com/doi/10.1002/pmic.201200463/abstract;jsessionid=A0EF1A8F9C5AB51A583E4E0B2CDA1AAA.f03t03 | ||
DATASET DESCRIPTION | ||
Proteomics lists |
Type: application/vnd.ms-excel
File size: 19 megabytes
Uploaded on: 2018-02-07 13:48:20