| STUDYID | STOREDB:STUDY1153 |
|---|---|
| CREATEDON | 2019-07-23 13:15:23 |
| MODIFIEDON | 2019-08-26 12:50:13 |
| UPLOADER | Daniela Hladik |
| DOI | DOI:10.20348/STOREDB/1153 |
| STUDY NAME | ||
|---|---|---|
| CREB signaling mediates dose-dependent radiation response in the murine hippocampus two years after low-dose total body exposure | ||
| STUDY STATUS | ||
| Published: Open access to everyone | ||
| COUNTRY | ||
| Germany | ||
| SPECIES | ||
| Mus musculus | ||
| SIZE OF COHORT | ||
| 0-999 | ||
| OUTCOME | ||
| Cognitive effects | ||
| INTERNAL OR EXTERNAL EXPOSURE | ||
| External | ||
| TYPE OF EXTERNAL EXPOSURE | ||
| gamma | ||
| RADIONUCLIDE | ||
| caesium | ||
| AGE AT EXPOSURE | ||
| Adult | ||
| EXPOSURE PATTERN | ||
| Acute | ||
| DOSE RATE | ||
| Low | ||
| BIOLOGICAL SAMPLE AVAILABLE | ||
| No | ||
| ECOLOGICAL DATA | ||
| No | ||
| STUDY DESCRIPTION | ||
| The impact of low-dose ionizing radiation (IR) on human brain has recently attracted attention due to increased use of IR for diagnostic purposes. The aim of this study was to investigate low-dose radiation response in hippocampus. Female C57Bl/6 mice were irradiated with 0, 0.063, 0.125 or 0.5 Gy and quantitative label-free proteomic analysis of hippocampus was performed after 24 months. Key pathways were validated by immunoblotting. CREB signaling and CREB-associated pathways were the most affected ones at all doses. The two lower doses seemed to induce CREB pathway, whereas the exposure to 0.5 Gy deactivated CREB. Similarly, the lowest dose (0.063 Gy) was anti-inflammatory reducing the number of activated microglia (IBA-1), whilst induction of activated microglia and reactive astroglia (GFAP) was found at 0.5 Gy suggesting increased inflammation and astrogliosis, respectively. Apoptotic markers BAX and cleaved CASP-3 and oxidative stress markers (carbonylation, NRF-2) were increased only at the highest dose. Since activated CREB pathway plays a central role in learning and memory, these data suggest neuroprotection at the lowest dose (0.063 Gy) but neurodegeneration at 0.5 Gy. These effects become significant only in old animals (24 m) and support the hypothesis of radiation-induced accelerated aging in the brain. | ||
| DATASET NAME | ||
|---|---|---|
| Raw files | ||
| DOI | DOI:10.20348/STOREDB/1153/1215 | |
| DATASET DESCRIPTION | ||
| MS spectral information | ||
| DATASET NAME | ||
|---|---|---|
| Lists of all identified proteins of the 4, 12 and 18 months timepoint | ||
| DOI | DOI:10.20348/STOREDB/1153/1214 | |
Type: application/vnd.ms-excel
File size: 6 megabytes
Uploaded on: 2019-08-13 13:34:20
| DATASET NAME | ||
|---|---|---|
| Table S1-S9 | ||
| DOI | DOI:10.20348/STOREDB/1153/1213 | |
| DATASET DESCRIPTION | ||
| LC-MS/MS raw data and filtered lists | ||
Type: application/vnd.ms-excel
File size: 5 megabytes
Uploaded on: 2019-08-13 13:34:56
Type: application/vnd.ms-excel
File size: 2 megabytes
Uploaded on: 2019-07-23 13:18:01
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