STOREDB:STUDY1169 X-ray induced bio-acoustic emissions from cultured cells [DOI:10.20348/STOREDB/1169]

Study meta-data


STUDYIDSTOREDB:STUDY1169
CREATEDON2021-06-13 22:02:20
MODIFIEDON2021-06-13 22:02:20
UPLOADERPaul Schofield
DOIDOI:10.20348/STOREDB/1169

Study details


STUDY NAME
X-ray induced bio-acoustic emissions from cultured cells
STUDY STATUS
Published: Open access to everyone
STUDY TYPE
09-22-00 - Acoustic radiation study
DATA SHARING POLICY
CC-Attribution Non-Commercial
COUNTRY
United Kingdom
PRINCIPAL INVESTIGATOR
Prof Carmel Mothersill
SPECIES
Homo sapiens
INTERNAL OR EXTERNAL EXPOSURE
External
TYPE OF EXTERNAL EXPOSURE
X-ray
EXPOSURE PATTERN
Acute
BIOLOGICAL SAMPLE AVAILABLE
No
ECOLOGICAL DATA
No
STUDY DESCRIPTION
We characterised for the first time the emission of bio-acoustic waves from cultured cells irradiated at doses of X-ray photon radiation relevant to medical and accidental exposure.
Methods and materials: Human cancer cell lines (MCF-7, HL-60) and control cell-free media were exposed to 1 Gy X-ray photons while recording the sound generated before, during and after irradiation. Cellular cytotoxicity following photon irradiation was determined by extracellular LDH levels, and irradiated cell conditioned media were tested for their ability to elicit a bystander effect in reporter cells.
We reported the first recorded acoustic signals captured from a collective pressure wave response to ionising irradiation. The signature of the collective acoustic peaks was : We show that at doses of X-ray irradiation capable of producing the release of bystander effect-inducing activity, both cell types emit a characteristic acoustic signal for the duration of the radiation pulse. The rapid signal decay is consistent with a passive rather than an active acoustic signal generation.
Data in this dataset comprise: Raw Picoscope outputs (CSV) for each experiment, Lactate dehydrogenase assay for each experiment as an excel file with plate arrangement, and relative mitochondrial membrane potentials for irradiated and control cells.

STOREDB:DATASET1245 Raw Picoscope data [DOI:10.20348/STOREDB/1169/1245]


Created on:2021-06-13 22:07:58
Modified On:2021-06-13 22:07:58
DATASET NAME
Raw Picoscope data
DOIDOI:10.20348/STOREDB/1169/1245
DATASET DESCRIPTION
Files comprise raw picoscope data as CSV files for each experimental group.
DMEM_IRRADIATED_4

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DMEM_IRRADIATED_5

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DMEM_IRRADIATED_3

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DMEM_IRRADIATED_3

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DMEM_IRRADIATED_1

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BACKGROUND MCF7 NO RADIATION 5

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BACKGROUND MCF7 NO RADIATION 4

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BACKGROUND MCF7 NO RADIATION 4

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BACKGROUND MCF7 NO RADIATION 2

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BACKGROUND MCF7 NO RADIATION 2

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BACKGROUND HL60 NO RADIATION 5

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BACKGROUND HL60 NO RADIATION 4

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BACKGROUND HL60 NO RADIATION 3

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BACKGROUND HL60 NO RADIATION 2

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BACKGROUND HL60 NO RADIATION 1

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RPMI_IRRADIATED_5

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File size: 497 megabytes

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RPMI_IRRADIATED_4

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File size: 696 megabytes

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RPMI_IRRADIATED_4

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RPMI_IRRADIATED_2

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RPMI_IRRADIATED_2

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MCF7_Irradiated_s4

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MCF7_Irradiated_s4

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MCF7_Irradiated_s3

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MCF7_Irradiated_s3

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HL60_Irradiated_s5

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HL60_Irradiated_s5

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HL60_Irradiated_s3

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HL60_Irradiated_s3

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HL60_Irradiated_s1

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HL60_Irradiated_s1

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STOREDB:DATASET1244 Lactate dehydrogenase assay [DOI:10.20348/STOREDB/1169/1244]


Created on:2021-06-13 22:06:25
Modified On:2021-06-13 22:06:25
DATASET NAME
Lactate dehydrogenase assay
DOIDOI:10.20348/STOREDB/1169/1244
DATASET DESCRIPTION
An hour after exposure culture media were collected and cleared by centrifugation at 100 x g at 5oC for 5 min to remove any remnant of cells. The LDH assays were performed in duplicate with 50 L conditioned cell culture media per well using the Abcam LDH (cytotoxicity) assay kit (#ab65393). The quantity of nicotinamide adenine dinucleotide (NADH) was assessed spectrophotometrically at 450 nm using the Tecan Infinite M200 Pro microplate reader with integrated command via the i-control® software and by mixing the NADH detection buffer with the supernatant according to the manufacturer’s instructions.
LDH Assay Radiation MCF7 HL60 - REACTION TIME VALUES

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File size: 56 kilobytes

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LDH Assay Radiation MCF7 HL60 - REACTION TIME VALUES

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STOREDB:DATASET1243 Mitochondrial membrane potential measurements [DOI:10.20348/STOREDB/1169/1243]


Created on:2021-06-13 22:04:23
Modified On:2021-06-13 22:04:23
DATASET NAME
Mitochondrial membrane potential measurements
DOIDOI:10.20348/STOREDB/1169/1243
DATASET DESCRIPTION
Cell culture conditioned medium (CCCM) and Irradiated cell culture conditioned medium (ICCM) were tested for their effects on the mitochondrial membrane potential of bystander reporter HCT116 p53+/+ cells. Briefly, reporter cells were exposed to CCCM or ICCM for 1 h at 37C. Then the reporter cells were incubated with the potentiometric fluorescent dye JC-1 to measure the relative mitochondrial membrane potential as previously described by Vo et al. (Vo et al. 2017). Relative fluorescent units were measured with the Tecan Infinite M200 Pro microplate reader with integrated command via the i-control® software.

Vo NTK, Sokeechand BSH, Seymour CB, Mothersill CE. 2017. Characterizing responses to gamma radiation by a highly clonogenic fish brain endothelial cell line. Environmental research.156:297-305. Epub 2017/04/05
Mitochondria membrane potentials

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File size: 15 kilobytes

Uploaded on: 2021-06-13 22:05:19