STOREDB:STUDY1169 X-ray induced bio-acoustic emissions from cultured cells [DOI:10.20348/STOREDB/1169]
|X-ray induced bio-acoustic emissions from cultured cells|
|Published: Open access to everyone|
|09-22-00 - Acoustic radiation study|
|DATA SHARING POLICY|
|Prof Carmel Mothersill|
|INTERNAL OR EXTERNAL EXPOSURE|
|TYPE OF EXTERNAL EXPOSURE|
|BIOLOGICAL SAMPLE AVAILABLE|
|We characterised for the first time the emission of bio-acoustic waves from cultured cells irradiated at doses of X-ray photon radiation relevant to medical and accidental exposure.
Methods and materials: Human cancer cell lines (MCF-7, HL-60) and control cell-free media were exposed to 1 Gy X-ray photons while recording the sound generated before, during and after irradiation. Cellular cytotoxicity following photon irradiation was determined by extracellular LDH levels, and irradiated cell conditioned media were tested for their ability to elicit a bystander effect in reporter cells.
We reported the first recorded acoustic signals captured from a collective pressure wave response to ionising irradiation. The signature of the collective acoustic peaks was : We show that at doses of X-ray irradiation capable of producing the release of bystander effect-inducing activity, both cell types emit a characteristic acoustic signal for the duration of the radiation pulse. The rapid signal decay is consistent with a passive rather than an active acoustic signal generation.
Data in this dataset comprise: Raw Picoscope outputs (CSV) for each experiment, Lactate dehydrogenase assay for each experiment as an excel file with plate arrangement, and relative mitochondrial membrane potentials for irradiated and control cells.
STOREDB:DATASET1245 Raw Picoscope data [DOI:10.20348/STOREDB/1169/1245]
Created on:2021-06-13 22:07:58 Modified On:2021-06-13 22:07:58
|Raw Picoscope data|
|Files comprise raw picoscope data as CSV files for each experimental group.|
STOREDB:DATASET1244 Lactate dehydrogenase assay [DOI:10.20348/STOREDB/1169/1244]
Created on:2021-06-13 22:06:25 Modified On:2021-06-13 22:06:25
|Lactate dehydrogenase assay|
|An hour after exposure culture media were collected and cleared by centrifugation at 100 x g at 5oC for 5 min to remove any remnant of cells. The LDH assays were performed in duplicate with 50 L conditioned cell culture media per well using the Abcam LDH (cytotoxicity) assay kit (#ab65393). The quantity of nicotinamide adenine dinucleotide (NADH) was assessed spectrophotometrically at 450 nm using the Tecan Infinite M200 Pro microplate reader with integrated command via the i-control® software and by mixing the NADH detection buffer with the supernatant according to the manufacturer’s instructions.|
STOREDB:DATASET1243 Mitochondrial membrane potential measurements [DOI:10.20348/STOREDB/1169/1243]
Created on:2021-06-13 22:04:23 Modified On:2021-06-13 22:04:23
|Mitochondrial membrane potential measurements|
|Cell culture conditioned medium (CCCM) and Irradiated cell culture conditioned medium (ICCM) were tested for their effects on the mitochondrial membrane potential of bystander reporter HCT116 p53+/+ cells. Briefly, reporter cells were exposed to CCCM or ICCM for 1 h at 37C. Then the reporter cells were incubated with the potentiometric fluorescent dye JC-1 to measure the relative mitochondrial membrane potential as previously described by Vo et al. (Vo et al. 2017). Relative fluorescent units were measured with the Tecan Infinite M200 Pro microplate reader with integrated command via the i-control® software.
Vo NTK, Sokeechand BSH, Seymour CB, Mothersill CE. 2017. Characterizing responses to gamma radiation by a highly clonogenic fish brain endothelial cell line. Environmental research.156:297-305. Epub 2017/04/05