Study meta-data

STUDYID	STOREDB:STUDY1236
CREATEDON	2026-03-30 15:30:25
MODIFIEDON	2026-03-30 15:30:25
UPLOADER	Simone Moertl
DOI	DOI:10.20348/STOREDB/1236

Study details

STUDY NAME
	Discover - Cerebellum
STUDY STATUS
	Restricted: Downloaders have to ask uploader for file access
DATA SHARING POLICY
	CC-Attribution Non-Commercial
COUNTRY
	Germany
PRINCIPAL INVESTIGATOR
	PD Dr. Simone Moertl
SPECIES
	Mus musculus
SIZE OF COHORT
	0-999
OUTCOME
	Cancer
RADONORM RESEARCH PRIORITY
	Analysis of mechanisms involved in low dose radiation through use and development of suitable cellular and animal models
EXPOSURE CONTEXT
	Medical
INTERNAL OR EXTERNAL EXPOSURE
	External
TYPE OF EXTERNAL EXPOSURE
	gamma
TYPE OF INTERNAL EXPOSURE
	gamma
AGE AT EXPOSURE
	Childhood
EXPOSURE PATTERN
	Acute
DOSE RATE
	High
BIOLOGICAL SAMPLE AVAILABLE
	No
ECOLOGICAL DATA
	No
STUDY DESCRIPTION
	Genome wide methylome and whole proteome profiling was performed on cerebellum samples collected from Ptch1⁺/⁻ mice following neonatal γ irradiation (0 Gy, 0.1 Gy, 2 Gy). Genomic DNA was extracted from frozen cerebellar tissue and analyzed by reduced representation bisulfite sequencing (RRBS). Reads were aligned to the reference genome using Bismark, and methylation levels at CpG sites were quantified. Differential methylation analysis was performed using the methylKit R package. The average methylation at each chromosome and at each annotated transcript are determined on a per-sample basis. For the region-based analysis, the genome is tiled into 1 kilobase regions and significantly differentially methylated tiles are identified (methylation difference > 20%). Proteins were extracted from frozen cerebellar tissue, digested, and analyzed by DIA-PASEF mass spectrometry. Data were processed using Spectronaut against the SwissProt mouse database. Differential protein expressions were defined based on q-value < 0.05, at least two unique peptides, and fold change ≥1.5 or ≤0.6.
MEAN DURATION OF FOLLOW-UP (WEEKS)
	6