STORE_DB

STOREDB:STUDY1086 Single Dose Fe Ion Irradiation Highlights the Radiation Quality Dependent Nature of the Endothelial Cell Response to Ionizing Radiation Exposure [DOI:10.20348/STOREDB/1086]

Study meta-data

STUDYID	STOREDB:STUDY1086
CREATEDON	2017-05-10 10:13:45
MODIFIEDON	2017-05-15 13:06:57
UPLOADER	Omid Azimzadeh
DOI	DOI:10.20348/STOREDB/1086

Study details

STUDY NAME
	Single Dose Fe Ion Irradiation Highlights the Radiation Quality Dependent Nature of the Endothelial Cell Response to Ionizing Radiation Exposure
STUDY STATUS
	Published: Open access to everyone

DATA SHARING POLICY
	CC-Attribution Non-Commercial

COUNTRY
	Germany

PRINCIPAL INVESTIGATOR
	Omid Azimzadeh
SPECIES
	Homo sapiens

SIZE OF COHORT
	0-999

OUTCOME
	Cardiovascular

RADONORM RESEARCH PRIORITY
	Analysis of mechanisms involved in low dose radiation through use and development of suitable cellular and animal models

EXPOSURE CONTEXT
	Occupational

INTERNAL OR EXTERNAL EXPOSURE
	External

TYPE OF EXTERNAL EXPOSURE
	X-ray

RADIONUCLIDE
	caesium

AGE AT EXPOSURE
	Adult

EXPOSURE PATTERN
	Acute

DOSE RATE
	High

BIOLOGICAL SAMPLE AVAILABLE
	Yes
STUDY DESCRIPTION
	Background and Purpose. Radiotherapy is an essential tool for cancer treatment. In order to spare normal tissue and to reduce the risk of normal tissue complications, particle therapy is a method of choice. Although a large part of the healthy tissue can be spared due to improved depth dose characteristics, little is known about the underlying biological and molecular mechanisms of particle irradiation in healthy tissues, such as the vasculature. Elucidation of these vascular effects is also required in the context of long term space flights, as particle radiation is the main contributor to the radiation effects observed in space. Endothelial cells, forming the inner monolayer of all vasculature are especially sensitive to irradiation and, if damaged, contribute to radiation-induced cardiovascular disease. Materials and Methods. Transcriptomics, proteomics and cytokine analyses were used to compare the response of endothelial cells irradiated with a single dose of X-ray or Fe ions (0 and 2.00 Gy) measured one and seven days post-irradiation. To support the observed inflammatory effects, endothelial cell adhesiveness to monocytes was assessed. Results. Our results indicate time- and radiation quality-dependent changes in the endothelial cell response upon irradiation. The irradiation impact was more pronounced and longer lasting for Fe ions than for X-rays. Both radiation qualities decreased the expression of genes involved in cell-cell adhesion and enhanced the expression of proteins involved in caveolar-mediated endocytosis signaling. Endothelial inflammation and adhesiveness was increased with X-rays, but decreased after Fe ion exposure. Conclusions. Fe ions induce pro-atherosclerotic processes in endothelial cells that are different in nature and/or kinetics than those induced by X-rays, highlighting the radiation quality-dependent differences in the induction and progression of cardiovascular diseases. Our findings give a better understanding of the underlying processes in endothelial cells after particle irradiation, a crucial aspect for the development of protective measures for cancer patients undergoing particle therapy as well for astronauts in space.
MEAN DURATION OF FOLLOW-UP (WEEKS)
	1

STOREDB:DATASET1126 EC 2Gy 1 day, 7 days [DOI:10.20348/STOREDB/1086/1126]

Created on:2017-05-10 10:14:18
Modified On:2017-05-10 10:14:18

DATASET NAME
	EC 2Gy 1 day, 7 days
DOI	DOI:10.20348/STOREDB/1086/1126
DATASET DESCRIPTION
	Background and Purpose. Radiotherapy is an essential tool for cancer treatment. In order to spare normal tissue and to reduce the risk of normal tissue complications, particle therapy is a method of choice. Although a large part of the healthy tissue can be spared due to improved depth dose characteristics, little is known about the underlying biological and molecular mechanisms of particle irradiation in healthy tissues, such as the vasculature. Elucidation of these vascular effects is also required in the context of long term space flights, as particle radiation is the main contributor to the radiation effects observed in space. Endothelial cells, forming the inner monolayer of all vasculature are especially sensitive to irradiation and, if damaged, contribute to radiation-induced cardiovascular disease. Materials and Methods. Transcriptomics, proteomics and cytokine analyses were used to compare the response of endothelial cells irradiated with a single dose of X-ray or Fe ions (0 and 2.00 Gy) measured one and seven days post-irradiation. To support the observed inflammatory effects, endothelial cell adhesiveness to monocytes was assessed. Results. Our results indicate time- and radiation quality-dependent changes in the endothelial cell response upon irradiation. The irradiation impact was more pronounced and longer lasting for Fe ions than for X-rays. Both radiation qualities decreased the expression of genes involved in cell-cell adhesion and enhanced the expression of proteins involved in caveolar-mediated endocytosis signaling. Endothelial inflammation and adhesiveness was increased with X-rays, but decreased after Fe ion exposure. Conclusions. Fe ions induce pro-atherosclerotic processes in endothelial cells that are different in nature and/or kinetics than those induced by X-rays, highlighting the radiation quality-dependent differences in the induction and progression of cardiovascular diseases. Our findings give a better understanding of the underlying processes in endothelial cells after particle irradiation, a crucial aspect for the development of protective measures for cancer patients undergoing particle therapy as well for astronauts in space.