STORE_DB

STUDY NAME
	Discover – MB Gene Expression
STUDY STATUS
	Restricted: Downloaders have to ask uploader for file access

STUDY TYPE
	09-07-00 - Gene expression study

DATA SHARING POLICY
	CC-Attribution Non-Commercial

COUNTRY
	Italy

PRINCIPAL INVESTIGATOR
	Simonetta Pazzaglia
SPECIES
	Mus musculus

SIZE OF COHORT
	0-999

OUTCOME
	Cancer

RADONORM RESEARCH PRIORITY
	Analysis of mechanisms involved in low dose radiation through use and development of suitable cellular and animal models

EXPOSURE CONTEXT
	Medical

INTERNAL OR EXTERNAL EXPOSURE
	External

TYPE OF EXTERNAL EXPOSURE
	gamma

AGE AT EXPOSURE
	Childhood

EXPOSURE PATTERN
	Acute

DOSE RATE
	High

BIOLOGICAL SAMPLE AVAILABLE
	Yes
ECOLOGICAL DATA
	No
STUDY DESCRIPTION
	Bulk RNA sequencing was performed on medulloblastoma samples collected from Ptch1⁺/⁻ mice following neonatal γ irradiation (0 Gy, 0.1 Gy, 2 Gy). Libraries were generated from total RNA extracted from freshly dissected tumors. Sequencing was carried out on an Illumina platform, producing paired end reads (150 bp). Samples included tumors arising spontaneously (0 Gy) or following low dose (0.1 Gy) or high dose (2 Gy) irradiation, with metadata specifying irradiation dose, latency, sex, and experimental batch. The dataset includes raw FASTQ files, and associated sample information, enabling downstream differential expression analyses and integrative comparisons across dose groups.
MEAN DURATION OF FOLLOW-UP (WEEKS)
	60

Created on:2026-03-25 13:32:30
Modified On:2026-03-25 13:32:30

DATASET NAME
	RNAseq_medulloblastoma
DOI	DOI:10.20348/STOREDB/1235/1356
TASK
	Task 1.1 Radiation-induced effects in the cerebellum
DELIVERABLE
	D2 Data set on SC-transcriptomic profiling, DNAmethylation and proteomic analysis in irradiated cerebellum; D3 Evaluating the role of microenvironment in radiation MB tumourigenesis
DATASET DESCRIPTION
	This dataset contains raw RNA sequencing data generated from medulloblastoma (MB) samples collected from Ptch1⁺/⁻ mice exposed to different doses of neonatal γ irradiation as part of the DISCOVER project. Tumors were harvested at endpoint and processed for bulk transcriptomic profiling. The dataset includes paired-end FASTQ files (150 bp) for pool of 3 tumor samples, accompanied by a standardized sample sheet specifying file paths and metadata. Each entry contains: sample identifier; R1 and R2 FASTQ file locations; library strandedness. A total of 12 tumor pool samples are included, spanning three radiation exposure groups: 0 Gy (0FA: 3 female spontaneous tumors; 0FB: 3 female spontaneous tumors; 0MA: 3 male spontaneous tumors); 0.1 Gy (1FA: 3 female low dose induced tumors; 1FB: 3 female low dose induced tumors; 1MA: 3 male low dose induced tumors; 1MB: 3 male low dose induced tumors ); 2 Gy (2FA: 3 female high dose induced tumors; 2FB: 3 female high dose induced tumors; 2MA: 3 male high dose induced tumors; 2MB: 3 male high dose induced tumors). All samples were sequenced on Illumina platforms.